e. estrogen (ER) and progesterone; Ki67 proliferation factor; cErb2 growth factor receptor), or apoptosis markers (Bcl2 and Bax). Biopsies (n = 55) of human ductal breast carcinoma (Jean-Perrin Anti-Cancer Center) and mammary tissues (n = 6) of healthy women (Edouard-Herriot Hospital), were used to investigate ZAG expression by immunohistochemistry (ABC technique, biotin-avidin-peroxidase). Statistical analysis was realized with Spearman correlation. ZAG expression was detected in ductal carcinoma and in normal epithelial adjacent tissue (87% and 94% of cases studied respectively) but was not found in normal tissue of healthy women. In cancer tissue, its expression was positively

correlated to leptin receptor (p = 0.01, r = 0.459) and negatively to adiponectin receptor (p = 0.03, BMN 673 cell line r = −0.371) and ER (p = 0.04, r = −0.279). We did not show statistically significant correlation between ZAG and the other studied markers. These preliminary results suggest both a close relationship between ZAG expression and pathways involving major adipokines or estrogen and, that ZAG may be a potential breast cancer biomarker, which Erismodegib research buy requires further investigations. 1 Caldefie-Chézet F, Biochem Biophys

Res Commun, 2005; 2 Jardé T, Proc Nutr Soc, 2008; 3 Hale LP, Clin Cancer Res, 2001. Poster No. 215 TP53 Mutations in CFDNA from Egyptian Patients, as Biomarkers for Cancer Prevention Gihan Hosny 1 , Pierre Hainaut2 1 Environmental Health & Molecular Carcinogenesis

Division, Dept of Environmental Studies, Institute of Graduate Studies and Research, University of Alexandria, Alexandria, Egypt, 2 Molecular Carcinogenesis, International Agency for Research on Cancer, Lyon, France Background: It is well known that chronic infections affect the microenvironment with a high proportion of cancer incidence.In Egypt, chronic infection with hepatitis C,HCV, is a widespread infection among Egyptian population, and has been associated with increased incidence of Hepatocellular Carcinoma,HCC, and in some studies Monoiodotyrosine with increased risk of non-Hodgkin lymphoma,NHL.P53 protein plays an important role in the maintenance of genome stability in mammalian cells;it acts in many processes including cell-cycle checkpoint, DNA repair, apoptosis, and angiogenesis. Mutations of P53 have been reported as common mutations in solid tumors,including HCC and NHL, and have been implicated in drug resistance, aggression and poor prognosis. Circulating free DNA(CFDNA) has been shown to be a good source of liver tissue derived DNA in African and Asian patients with chronic liver disease or HCC. Objective: We have examined the presence of p53 mutations from exons 5 to 9 in CFDNA of patients with HCC or chronic liver disease, and of patients with NHL from Alexandria,Egypt, in two separate case-control studies.