Finally, the association of p16 and miR-29b expression was assessed. Overall, no significant

association was found between any of the tested microRNAs and survival, with the exception of miR-21 for which a deleterious prognostic effect of lowered expression was suggested. Otherwise, no single or combinatorial microRNA expression profile predicted response to adjuvant cisplatin-based chemotherapy. Together, our results indicate that the microRNA expression patterns examined were neither predictive nor prognostic in a large patient cohort with radically resected NSCLC, randomized to receive adjuvant cisplatin-based chemotherapy versus follow-up only. Cancer Res; 70(21); 8288-98. (C) 2010 AACR.”
“Living cells are constantly subjected to various mechanical LB-100 in vitro stimulations. They must find more sense the mechanical aspects of their environment and respond appropriately for proper cell function. In general, cells adhere to substrata. Thus, the cells must receive and respond to mechanical stimuli mainly from the substrata. For example, migrating cells can create their own polarity and migrate in a certain direction even in the absence of any attractive

substance. In order to generate such polarity, cells must sense mechanical stimuli from the substrata and transduce these stimuli into intracellular signals. To investigate the relationship between signals derived from mechanical stimuli and related cell functions, one of the most commonly used techniques

is the application of mechanical stimuli via stretching of elastic substrata. Here, we developed a new stretching device using a shape-memory alloy (SMA). An SMA has three advantages as an actuator of stretching devices: (i) the cost of the SMA required for the device is inexpensive, HIF inhibitor similar to$30 USD, (ii) the size of an SMA is very small (0.62 mm in diameter and 22 mm in length), and (iii) an SMA does not generate any vibrating noise, which can negatively affect cells. In response to the cyclic stretching by the new stretching device, Dictyostelium discoideum cells migrated perpendicular to the stretching direction and the migrating speed increased significantly. To our knowledge, this is the first report indicating that migrating cells can create their own polarity by the mechanical stimuli from the substrata.”
“Blood-borne tissue factor (TF)-bearing microparticles have been shown to play an important role in thrombus propagation in experimental models. The pathophysiologic role of these microparticles is being investigated in several prothrombotic conditions including cancer-associated thrombosis. Tumor cells are known to shed TF-bearing microparticles in vitro, and circulating TF-bearing microparticles can be measured in plasma samples from patients with advanced cancer.

35 mg/g). This difference was mainly because of the accumulation of liver triglycerides in the oxaliplatin group. CONCLUSIONS:

The current results indicated that C57BL/6 mice receiving weekly oxaliplatin can be used as a model for CASH. Oral FABAC therapy reduced the development of CASH in animals that received oxaliplatin. To the authors’ knowledge, this report is the first description of a model and a potential preventive treatment for CASH. Cancer 2010;116:251-5. (C) 2070 American DMXAA mouse Cancer Society.”
“To assess the activity of ceftolozane, a novel oxyimino-cephalosporin, in comparison with ceftazidime and piperacillin/tazobactam against a multidrug-resistant Pseudomonas aeruginosa strain using a murine model of pneumonia.\n\nQuantitative bacteriology, survival, histological examination, myeloperoxidase activity, proinflammatory cytokine levels in lungs and endothelial permeability were evaluated to determine the effects of ceftolozane and comparators on P. aeruginosa-induced pneumonia.\n\nAfter 48 h of treatment, ceftolozane reduced the bacterial load by 34 log(10) cfu/g of lung. Systemic dissemination

of the pulmonary infection and development of lung damage were inhibited in all -lactam-treated animals. P. aeruginosa-induced pneumonia led to elevated concentrations of tumour necrosis factor-, interleukin (IL)-1 and macrophage inflammatory protein (MIP)-2 in the lungs. While the levels of proinflammatory cytokines decreased following ceftazidime and piperacillin/tazobactam compound screening assay therapy, MG-132 ceftolozane exhibited increased concentrations of IL-1 and MIP-2 after 24 h of infection, resulted in significantly increased levels of recruited neutrophils within the infected lung without increasing lung endothelial permeability.\n\nThese data strongly support ceftolozane as an effective option for the treatment of severe P. aeruginosa respiratory infections by improving the early pulmonary inflammatory response without impairing 48 h post-infection homeostasis.”
“Aims: Considering the sparse information about the clinical utility of the novel immunohistochemical marker ProEx C in histological sections, a decision

was taken to study the pattern of ProEx C expression in normal/benign cervical epithelium (N/B), low-grade squamous intraepithelial lesion (LGSIL) and high-grade squamous intraepithelial lesion (HGSIL), as well as the association of ProEx C expression with human papillomavirus (HPV) genotypes.\n\nMethods: 100 cervical samples, including 21 N/B cervices, 16 LGSILs, 61 HGSILs and two cervical invasive carcinomas, were obtained from conisation and hysterectomy. Surgical specimens were arranged in three tissue microarrays and stained for ProEx C. Ninety-three samples were HPV genotyped. Genotyping was performed by DNA amplification and hybridisation with genotype-specific probes on a low-density DNA array.\n\nResults: ProEx C-positive expression in more than the lower third of the epithelium was observed in 14.3% of N/B, 62.

Although the pericardium was opened, left ventricular function remained depressed, necessitating extracorporeal membrane oxygenation, which was withdrawn after

48 h of assistance when left ventricular function had recovered. The patient was extubated on the seventh postoperative day but died of multiorgan failure on the 64th postoperative day.”
“The World Health Organization (WHO) issued a recommendation (Technical Consultation: Paris 2006, Luxembourg 2007) that salt consumption, as a risk factor for hypertension, atherosclerosis, myocardial infarction, stroke, and select cancers, should be restricted. The European Commission looked to adhere to this recommendation by creating the High Level Croup on Nutrition and Physical Activity. According to WHO\n\nrecommendations, a daily allowance of Selleckchem 3-deazaneplanocin A 5 g NaCl (i.e., Cyclopamine inhibitor 2 g Na) for individual salt consumption should not be exceeded. At present, mean individual salt consumption in Poland totals 13.5 g, of which salt used in household constitutes 8.8 g. In some regions of Poland, this number reaches upwards of 15.0 g/person. The Position Paper on Initiatives

Aimed at Decreasing Salt Consumption in Poland, developed by an expert group at the National Food and Nutrition Institute, set the course for intervention, including changing recipes for mass-produced food products and large-scale catering, improving oversight by food control agencies, and continuing legislative changes. These interventions should also include education directed towards consumers, food producers, public health professionals, healthcare workers, and media representatives. The Position Paper of the Polish

Hypertension Society also sets the course for promoting restricted salt consumption and controlling hypertension on a population level. However, household PFTα chemical structure salt is the main carrier of iodine in the Polish model of iodine prophylaxis. Thus, any interventions also require synchronized action with the Polish Council for Control of Iodine Deficiency Disorders. Current efforts aimed at preventing iodine-deficiency look to increase consumption of other iodine-rich products (e.g., milk, mineral water) with standardized levels of iodine. Once they achieve an iodine concentration of 0.1-0.2 mg, these products can easily supplement any decrease in physiological iodine levels resulting from reduced salt consumption. Also required are wide-ranging educational campaigns which will be coordinated by the new designated WHO Collaborating Centre for Nutrition at the Chair of Endocrinology at Jagiellonian University, Collegium Medicum in Krakow.

“
“Nuclear uptake of the simian virus (SV) 40 T antigen is triggered by a specific nuclear localization sequence. However, such a nuclear localization sequence is only poorly taken up by the cytoplasm of cells when administered to the culture medium. Our aim was to improve the cytoplasmic uptake of the SV 40 T antigen nuclear localization Adavosertib in vivo sequence. Consequently, we synthesized novel

fluorescein isothiocyanate-labelled conjugates containing the nuclear localization sequences of the SV 40 T antigen and either trichlorobenzoic or trifluorobenzoic acid. Applied at 260 mu m such halogenated NLS conjugates were nuclearly taken up by 75-85% of U373 and LN18 glioma cells and resulted in cell death. Nuclear staining and cell death were also found at lower concentrations (130 and 65 mu m) of halogenated nuclear localization sequence conjugates. By contrast only a low cellular staining rate and no cell death could be observed after co-incubation with a trichlorobenzoic acid or trifluorobenzoic acid-lacking nuclear localization sequence conjugate and free, unbound trichlorobenzoic acid or trifluorobenzoic acid at the high concentration (260 mu m). Such small non-radioactive fluorinated

and chlorinated nuclear localization sequences may be used as important components for future antiglioma drug development.”
“Background: Telomere length, an indicator of ageing and longevity, has been correlated with several biomarkers of cardiometabolic disease in both Arab children and adults. It is not known, however, whether or not telomere THZ1 mw length is a highly conserved inheritable trait in this homogeneous cohort, where age-related diseases are highly prevalent. As such, the aim of this study was to address the inheritability of telomere length in

Saudi families and the impact of cardiometabolic disease biomarkers on telomere length.\n\nMethods: learn more A total of 119 randomly selected Saudi families (123 adults and 131 children) were included in this cross-sectional study. Anthropometrics were obtained and fasting blood samples were taken for routine analyses of fasting glucose and lipid profile. Leukocyte telomere length was determined using quantitative real time PCR.\n\nResults: Telomere length was highly heritable as assessed by a parent-offspring regression [h2 = 0.64 (p = 0.0006)]. Telomere length was modestly associated with BMI (R-2 0.07; p-value 0.0087), total cholesterol (R-2 0.08; p-value 0.0033), and LDL-cholesterol (R-2 0.15; p-value 3 x 10(-5)) after adjustments for gender, age and age within generation.\n\nConclusion: The high heritability of telomere length in Arab families, and the associations of telomere length with various cardiometabolic parameters suggest heritable genetic fetal and/or epigenetic influences on the early predisposition of Arab children to age-related diseases and accelerated ageing.

Additionally, certain breast cancer biomarkers are fucose-rich while a well-known marker of breast cancer progression, soluble E-selectin, is a known counter-receptor of fucosylated selectin ligands. We provide illustrative examples and supportive evidence drawn from work with human breast

cancer cell lines in vitro as well as clinical studies with human pathologic material. And finally, we discuss evidence that fucose (or its absence) is central to the mechanisms of action of several experimental targeted therapies which may prove useful in breast cancer treatment. We propose that alpha-L-fucose is essential in order to construct first, the malignant and then the metastatic phenotype of many human breast cancers. This knowledge may inform the search Selleck IPI-145 for novel treatment approaches in breast cancer.”
“Objective To qualitatively and quantitatively assess the presentation of hepatic focal nodular hyperplasia (FNH) at diffusion-weighted magnetic resonance imaging (DWMRI) using multiple high b values. Materials and Methods Twenty-five patients with 27 FNHs

had liver DWMRI at 1.5 T using free-breathing acquisition and 3 b values (0, 600, 1000 s/mm(2)). Focal nodular hyperplasias were evaluated qualitatively using visual analysis of diffusion-weighted magnetic resonance (DWMR) images and quantitatively using conventional apparent diffusion coefficient (ADC) and normalized ADC Batimastat concentration measurements. Results All FNHs (100%) were visible on b(0) DWMR images; 26 of the 27 FNHs (96%), on b(600) DWMR images; and 21 of the 27 FNHs (78%), on b(1000) DWMR images. A total of 18 of the 27 FNHs (67%) exhibited a hyperintense central scar on the b(0) DWMR images that remained visible on the b(600) and b(1000) DWMR images in 6 of the 27 FNHs (22%). Conventional ADC value of FNHs (1.318 x 10(-3) mm(2)/+/- 0.208) was significantly lower than that of adjacent hepatic parenchyma (1.414 x 10(-3) mm(2)/s +/- Autophagy Compound Library manufacturer 1.95) (P = 0.0003), although a substantial overlap was

found. The use of normalized ADC using the liver as reference organ resulted in a more restricted distribution of ADC values (variation coefficient, 5.3%). Conclusions Focal nodular hyperplasias show a wide range of morphological features at DWMRI using high b values. Further studies are needed to fully investigate as to what extent normalized ADC may result in better lesion characterization.”
“Atmospheric ozone plays an important role in understanding the processes occurring in the atmosphere and changes in the climate. This article relates to experimental results from balloon-borne research in the stratosphere for the study of chemical and dynamical processes influencing climate change and for validation of satellite observations. Total ozone observations in western Siberia were performed by the Brewer MKIV S/N 049 spectrophotometer in Tomsk and the SAOZ UV-Vis spectrometer in Salekhard.

Recollected procedural pain scores at 24 hours after surgery were not statistically different between groups. Median scores on the Wong-Baker FACES pain scale for the 2 groups were 2.0 (interquartile range, 3.1) for the fentanyl-first group and 1.5 (interquartile range, 2.5) for the midazolam-first group (P = .333). There was no statistical difference in the change in vital signs from baseline to 2 surgical end points in the 2 groups. In addition, patient satisfaction with the procedure did not statistically differ between the 2 groups. Conclusions: In this study,

selective sequencing of midazolam or fentanyl during Selleckchem AG-14699 an intravenous moderate-sedation procedure did not result in a measurable difference of recollected procedural pain scores at 24 hours after third molar extraction. The choice of the sedation agents and the order of their administration should be tailored to the patient’s needs, type of surgical procedure, and surgeon preference. (C) 2015 American Association of Oral

and Maxillofacial Surgeons”
“This SYN-117 study investigated the effects of combined teriparatide (an anabolic agent) and monthly risedronate (an anti-resorptive agent) therapy on cancellous bone mass in orchidectomized (ORX) rats. Fifty 14-week-old male Sprague-Dawley rats were randomized into five groups of ten animals each: sham-operation + vehicle; ORX + vehicle; ORX + risedronate (90 mu g/kg subcutaneous, every 4 weeks); ORX + teriparatide (30 mu g/kg subcutaneous, three times per week); and ORX + risedronate + teriparatide. After the 12-week experimental period, cancellous bone in the tibial proximal metaphysis was examined by static and dynamic histomorphometric analyses. ORX decreased bone volume

per total volume (BV/TV) and trabecular number (Tb.N), and increased trabecular separation (Tb.Sp). Risedronate increased BV/TV and Tb.N above the sham control values, while teriparatide prevented the ORX-induced decrease in BV/TV and increased trabecular width (Tb.Wi) above sham control levels. Risedronate decreased Tb.Sp below control values, while teriparatide prevented the ORX-induced increase in Tb.Sp. The combination of teriparatide and risedronate further increased BV/TV EPZ5676 molecular weight and Tb.N and decreased Tb.Sp as a result of suppression of bone remodeling, compared with teriparatide alone. These results suggest that teriparatide and monthly risedronate exert different effects on cancellous bone structure and thus have additive effects on cancellous bone mass in ORX rats.”
“Emerging evidence indicates that chronic inflammation plays an important role in prostate carcinogenesis. Yet to date the precise molecular and cellular mechanisms linking inflammation to carcinogenesis remains unclear. The purpose of this study was to determine the local contribution of prostate epithelial cells to the inflammatory process.

The conjugate contains six L-lysine

units with five INH molecules, which are attached by means of pH-sensitive amidine bond. Under in vitro conditions, the conjugate is hydrolyzed and isoniazid is released (pH 4; 37 degrees C; t(1/2) approximate to 10 h). (C) 2012 Elsevier Ltd. All rights reserved.”
“Linear nevus sebaceous syndrome is reported to occur in as many as I in 1000 live births, and is hypothesized to result from genetic mosaicism selleck involving a lethal autosomal-dominant gene. The terms “epidermal nevus syndrome” and “linear nevus sebaceous syndrome” are often used interchangeably, although linear nevus sebaceous syndrome more strictly applies to patients with a typical midline nevus, and epidermal nevus syndrome is a more inclusive term that includes all varieties of epidermal nevi. Linear nevus sebaceous syndrome encompasses a broad spectrum of abnormalities that may affect every organ system, including the central nervous system. In these cases, seizures and mental retardation are the main manifestations. Many other organ systems were also reported to be involved in this syndrome,

including the cardiovascular, skeletal, ophthalmologic, and urogenital systems, among others. Although linear nevus sebaceous syndrome occurs at a relatively high frequency and may affect different organ systems, many physicians are unaware of the syndrome, which may delay diagnosis and treatment. We present two cases this website of linear nevus sebaceous syndrome, and we describe their initial presentation and subsequent evolution, incorporating a review of the current literature in this field. (C) 2008 by Elsevier Inc. All rights reserved.”
“Objectives The aim of this study was to evaluate Daporinad chemical structure the association between calculated creatinine clearance (CCC)-based contrast dose and renal complications in patients undergoing percutaneous coronary interventions (PCI).\n\nBackground Excess

volumes of contrast media are associated with renal complications in patients undergoing cardiac procedures. Because contrast media are excreted by the kidney, we hypothesized that a dose estimation on the basis of CCC would provide a simple strategy to define a safe dose of contrast media.\n\nMethods We assessed the association between CCC-based contrast dose and the risk of contrast-induced nephropathy (CIN) and need for in-hospital dialysis in 58,957 patients undergoing PCI and enrolled in the BMC2 (Blue Cross Blue Shield of Michigan Cardiovascular Consortium) registry from 2007 to 2008. Patients receiving dialysis at the time of the procedure were excluded.\n\nResults The risk of CIN and nephropathy requiring dialysis (NRD) was directly associated with increasing contrast volume adjusted for renal function. The risk for CIN and NRD approached significance when the ratio of contrast dose/CCC exceeded 2 (adjusted odds ratio [OR] for CIN: 1.16, 95% confidence interval [CI]: 0.98 to 1.37, adjusted OR for NRD: 1.

(C) 2015 Elsevier Inc. All rights reserved.”
“Objective: To investigate the prevalence of Kidd antigens among pregnant women in Sokoto, North Western Nigeria. Methods: One hundred and sixty two pregnant women aged 18-45 years [mean age (27.19 +/- 4.72) years] VS-4718 cost attending antenatal clinic in Usmanu Danfodiyo University Teaching Hospital, Sokoto, were screened for the presence of Kidd blood group antigens using the conventional tube method and anti-Jka and Jkb reagents (Lorne Laboratories, UK). Results: Out of the 162 pregnant women tested, 82 (50.6%) were Hausa, 2606%’ were Igbo, 23 (14.2%) were Fulani and 20 (12.3%) were Yoruba while the minority ethnic groups were 11 (6.8%).

The distribution of Kidd antigen was compared based on the ethnic groups of subjects. Jka antigen was the highest among the Yoruba ethnic group (10.0%.) followed by the Hausa ethnic group (7.31%). The prevalence of Jkb was highest among Hausa subjects (10.97%) followed by the Yoruba ethnic group

(10.0%). Subjects were categorized based on parity. Majority of the subjects were rnultigravidae, 122 (75.3%) compared to primigravidae 40 (24.7%). Subjects were stratified based on trimester. A significant number of women were in the second trimester, 111 (68.5%) compared to the third trimester 38 (23.5%) and the first 13 (8.0%). The distribution SYN-117 price of Kidd antigens among subjects studied indicated a prevalence of Jka, Jkb and Jk(a+b+) with 8(4.9%). 13(8.0%) and 0(0.0%). respectively. A significant number of subject tested were negative for Kidd antigens. Of the 162 pregnant women tested, 154 (95.1%), 149 (75.3%) and 141 (87.04%) tested were negative for Jka, JO), and Jko(a-b-), respectively. Conclusions: This study indicates that blood group antigens can be distributed differently within different nationalities. Kidd phenotypes observed among pregnant women in this study was similar to previous reports BYL719 datasheet among blacks but at variance with report among Caucasians and Asians. We recommend that detailed routine phenotyping for all clinically significant red cell antigen including Kidd antigen

being earned out routinely among all pregnant women in Nigeria. There is also the need to routinely screen all pregnant women for alloantibodies to facilitate the selection of antigen negative units for those with clinically significant alloantibodies who require a red cell transfusion. This can potentially optimise the obstetric management of haemolytic disease of foetus and newborn and prevent haemolytic transfusion reaction among pregnant women.”
“Purpose\n\nWe performed a case-control genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with musculoskeletal adverse events (MS-AEs) in women treated with aromatase inhibitors (AIs) for early breast cancer.\n\nPatients and Methods\n\nA nested case-control design was used to select patients enrolled onto the MA.

57.0%; P < 0.01), and with CL at G1 (94.0% vs. 67.8%; P < 0.01). Double-Ovsynch also increased the percentage of cows with high P4

(>3.0 ng/mL) at PCF2 alpha (88.0% vs. 76.3%; P = 0.04) and tended to increase average circulating P4 at PGF(2 alpha) (3.52 +/- 0.17 ng/mL vs. 3.09 +/- 0.21 ng/mL; P = 0.11). Double-Ovsynch also tended to increase percentage of cows ovulating to G1 (80.0% vs. 69.9%; P = 0.11) and G2 (98.0% vs. 93.5%; P = 0.08). Thus, presynchronization of cows with Double-Ovsynch induced ovulation in noncycling cows and appeared to increase most aspects of synchronization during the Ovsynch protocol. (C) 2013 Elsevier Inc. All selleck chemical rights reserved.”
“Magnetoacoustic detection is a new method for the noninvasive, early detection of cancer. It uses specific superparamagnetic nanoparticles (NPs) that bind to tumor sites together with magnetic excitation and acoustic detection of the tumor-NPs complex. This work

tests the feasibility of such method theoretically and experimentally. An extensive analytic model has been developed that shows an ability to detect small tumors, a few centimeters deep inside the tissue. A series of experiments were conducted to validate PKC412 manufacturer the theoretical model. The performance of specially designed solenoids was measured, and the detection of the tumor presence in phantom was demonstrated. Experimental results agree well with the theoretical calculations, providing preliminary proof of concept. We demonstrate the ability to detect a 5-mm diameter spherical tumor located 3 cm deep. Instrumentation and measurements are inexpensive and accurate. The accuracy, speed, and costs of this method show the potential for early detection of cancer.”
“Neurologic emergencies

are common, frequently www.selleckchem.com/products/netarsudil-ar-13324.html devastating, and benefit from timely diagnosis and treatment. Point of care (POC) technologies have the potential to assist clinicians caring for these patients. In order to prioritize development of new POC testing, a thorough assessment of clinical needs is required. We describe the methods of the clinical needs assessment (CNA) process and provide the initial findings of a CNA for POC technologies in neurologic emergencies performed to support a National Institute of Biomedical Imaging and Bioengineering (NIBIB) initiative.\n\nCNA is an iterative process. An initial survey instrument was developed through consensus by a multi-disciplinary panel and underwent internal validation through beta-testing and face-validity assessment. This survey was distributed at the national meetings of several academic medical societies and results were used to redesign of the survey tool for broader distribution. Analysis of responses from the revised survey supported the release of a request for proposals (RFP) in 2010. Survey revision continues, and expanded CNA efforts with focus groups are being designed in anticipation of another RFP in 2012.\n\nThe initial survey identified six areas of clinical need and two domains of interest.

Mycoplasma pneumoniae DNA was detected in serum from 10 patients with RT-PCR. Legionella pneumophila urinary antigen was detected in 5 patients. Serological IgM antibodies to Chlamydia pneumoniae in 7 patients and Respiratory Syncytial Virus in 2 patients were observed. Etiology was not determined in 32.5% of patients. The most frequently identified pathogens causing CAP were S. pneumoniae, M. pneumoniae, and C. pneumoniae in descending order in our hospital.\n\nConclusion: Although determination of causative agents in all CAP patients has not been accomplished, knowledge of the spectrum and frequency of local causative

agents are valuable for targeted therapy.”
“Objective: To highlight the possible association of intracranial aneurysm with autosomal MI-503 recessive

polycystic kidney disease.\n\nDesign, Setting, and Patient: To our knowledge, this association has been reported only twice LXH254 in vivo in the medical literature. We herein report the case of a 21-year-old man with autosomal recessive polycystic kidney disease, presenting with subarachnoid hemorrhage secondary to a ruptured intracranial aneurysm, at our institution.\n\nResults: In the presence of only 3 cases in the medical literature, one might conclude they are a simple coincidence. However, should this association exist, such as with the dominant form, then the neurologic prognosis and even the life of young patients may be at stake.\n\nConclusions: Given the devastating consequences of intracranial bleeding in young patients, early neurologic screening may be warranted. JAMA Neurol. 2013;70(1):114-116. Published online October 1, 2012. doi:10.1001/jamaneurol.2013.584″
“Objective.

Until now studies concerning the involvement of hepatic nonparenchymal cells (NPCs), particularly Kupffer cells/macrophages (KCs/MPs), in the pathogenesis of human nonalcoholic steatohepatitis learn more (NASH) have been limited to adult patients; there are no similar reports referring to children. This study aimed to explore, based on ultrastructural analysis, the role of KCs/MPs in the morphogenesis of nonalcoholic steatohepatitis (NASH) in children. Material and methods. Ultrastructural investigations of KCs were conducted on liver bioptates obtained from 10 children, aged 2-14 years, with clinicopathologically diagnosed NASH. Bioptatic material was fixed in solution of paraformaldehyde and glutaraldehyde in cacodylate buffer, routinely processed for transmission-electron microscopic analysis and examined using an Opton EM microscope. Results. The current ultrastructural study revealed within the hepatic sinusoids the presence of numerous enlarged KCs with increased phagocytic activity, which reduced or blocked vascular lumen. Interestingly, the activated KCs not only contained primary and secondary lysosomes, altered mitochondria, and well-developed Golgi apparatus, but also absorbed fragments of erythrocytes.