At each sampling point, LB agar was pre-contaminated with A. baumannii M3237 suspension to obtain surface concentrations
of 5 × 101, 5 × 102, and 5 × 103 CFU/ml. Contaminated agar plates were dried for 30 min in a biosafety hood at room temperature and divided into two groups: test agars received 0.1 or 0.5 ml of the phage-containing lotion to simulate the volumes of lotion used by most hand cream consumers and control. The control agars consisted of a phage-free lotion. The OSI-906 ic50 test and control agars were then incubated for 24 h at 37°C, and bacterial recovery counts calculated by comparing the number of A. baumannii M3237 colonies from the test agars with those from the control agars. ϕAB2 in glycerol as a hand sanitizer Briefly, the phage stock was mixed with glycerol to obtain a solution of 10% (v/v) glycerol/108 PFU/ml phage and stored at room temperature for up to 180 days to obtain a kinetic curve of the phage variation during this period. Phage stability and ability to inhibit A. baumannii M3237 was determined as described above for lotions. Statistical analysis Statistical analyses
were performed using SPSS, version click here 17.0 (SPSS Institute Inc., Chicago, IL, USA). Measurement of ϕAB2 bactericidal effect in liquid suspensions and glass slides, comparison of A. baumannii M3237 survival rates with different incubation times and control sets and reduction of viable A. baumannii M3237 by ϕAB2 lotion or glycerol was performed using one-way ANOVA, followed by Tukey’s test. Acknowledgments We thank Prof. Yi-Hsiung Tseng for critical reading of our manuscript. This work was
supported by grant NSC 100-2314-B-320-003 from the National Science Council, Republic of China; grant TCSP99-03-05 from Buddhist Tzu Chi General Hospital; and grant TCIRP98003-03 from Tzu Chi University. Yu-Lin Liu was supported by a graduate scholarship from the latter grant during part of this research project. References 1. Bergogne-Berezin see more E, Towner KJ: Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996, 9:148–165.PubMed 2. Villegas MV, Hartstein AI: Acinetobacter outbreaks, 1977–2000. CP673451 order Infect Control Hosp Epidemiol 2003, 24:284–295.PubMedCrossRef 3. Okpara AU, Maswoswe JJ: Emergence of multidrug-resistant isolates of Acinetobacter baumannii . Am J Hosp Pharm 1994, 51:2671–2675.PubMed 4. Gaynes R, Edwards JR: Overview of nosocomial infections caused by gram-negative bacilli. Clin Infect Dis 2005, 41:848–854.PubMedCrossRef 5. Meric M, Kasap M, Gacar G, Budak F, Dundar D, Kolayli F, Eroglu C, Vahaboglu H: Emergence and spread of carbapenem-resistant Acinetobacter baumannii in a tertiary care hospital in Turkey. FEMS Microbiol Lett 2008, 282:214–218.PubMedCrossRef 6.