Conserv Biol 2009, in press. 49. Van Doninck K, Schon I, Martens K, Backeljau T: Clonal diversity in the ancient asexual ostracod Darwinula stevensoni assessed by RAPD-PCR. Heredity 2004,93(2):154–160.CrossRefPubMed 50. Drouin G, de Sa MM: The concerted evolution KU55933 molecular weight of 5S ribosomal
genes linked to the repeat units of other multigene families. Mol Biol Evol 1995,12(3):481–493.PubMed 51. Vralstad T, Knutsen AK, Tengs T, Holst-Jensen A: A quantitative TaqMan MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci. Vet Microbiol 2009,137(1–2):146–155.CrossRefPubMed 52. Betsou F, Beaumont K, Sueur JM, Orfila J: Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples. J Clin Microbiol 2003,41(3):1274–1276.CrossRefPubMed 53. Gregory JB, Litaker RW, Noble RT: Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples. Appl RG7112 concentration Environ Microbiol 2006,72(6):3960–3967.CrossRefPubMed 54. Bart A, Heijden HM, Greve S, Speijer D, Landman WJ,
van Gool T: Intragenomic variation in the internal transcribed spacer 1 region of Dientamoeba fragilis as a molecular epidemiological marker. J Clin Microbiol 2008,46(10):3270–3275.CrossRefPubMed 55. Worheide G, Nichols SA, Goldberg J: Intragenomic variation of the rDNA internal transcribed spacers in sponges (phylum Porifera ): implications for phylogenetic studies. Mol Phylogenet Evol 2004,33(3):816–830.CrossRefPubMed
Prostatic acid phosphatase 56. Papin JF, Vahrson W, Dittmer DP: SYBR Green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain variability. J Clin Microbiol 2004,42(4):1511–1518.CrossRefPubMed 57. Anderson TP, Werno AM, Beynon KA, Murdoch DR: Failure to genotype herpes simplex virus by real-time PCR assay and melting curve analysis due to sequence variation within probe binding sites. J Clin Microbiol 2003,41(5):2135–2137.CrossRefPubMed 58. Lind K, Ståhlberg A, Zoric N, Kubista M: C646 Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis. Biotechniques 2006,40(3):315–319.CrossRefPubMed 59. Reischer GH, Kasper DC, Steinborn R, Mach RL, Farnleitner AH: Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions. Appl Environ Microbiol 2006,72(8):5610–5614.CrossRefPubMed 60. Blazer VS, Vogelbein WK, Densmore CL, May EB, Lilley JH, Zwerner DE:Aphanomyces as a cause of ulcerative skin lesions of menhaden from chesapeake bay tributaries. J Aquat Anim Health 1999,11(4):340–349.CrossRef 61.