Copper content went up

after treatment by copper nanoparticles in roots (by 94%); however, in selleck compound leaves, it Dorsomorphin ic50 decreased (by 38%). The content of manganese increased (by 30%) in leaves of treated plants and remained at control level in the roots. Figure 1 Content of metal elements in wheat seedling tissues after treatment with individual metal nanoparticles. 1 – roots, control; 2 – roots, experiment; 3 leaves, control; 4 – leaves, experiment. Thus, the results indicate the ability of metal nanoparticles to penetrate through the seed coat. The distribution of elements in plant tissues is determined by their ability to penetrate and peculiarities of transporting in the plant. Concerning the mechanism of processes, we could assume that nanoparticles with diameter less than the pore diameter of the cell wall could easily pass through and reach the plasma membrane [9]. After entering the cells, the nanoparticles transport from one cell to another through plasmadesmata. Major cell wall components are carbohydrates which are linked to form a rigid complex network and proteins [10]. The functional groups, such as carboxylate, phosphate, hydroxyl, amine, sulfhydryl, and imidazole, contained in these biomolecules offer a range of distinct active sites [11]. We investigated both the

mixtures of nanoparticle solutions and the way of their application (pre-sowing treatment and spraying of aboveground plant parts) impact upon metal contents in plant roots and leaves (aboveground parts) (Figures 2,3,4 and 5). selleck screening library Figure 2 Content of iron in wheat seedling tissues. Iron content in tissues after treatment of seeds (a) and leaves (b) with the mixture of metal nanoparticles: 1 – roots, control; 2 – roots, experiment; 3 – leaves, control; 4 – leaves, experiment. Figure 3 Content of copper in wheat seedling tissues. Copper content in tissues after treatment of seeds (a) and leaves (b) with the mixture of metal nanoparticles: 1 – roots, control; 2 – roots, experiment;

3 – leaves, control; 4 – leaves, experiment. Figure 4 Content of manganese in wheat seedling tissues. Manganese Coproporphyrinogen III oxidase content in tissues after treatment of seeds (a) and leaves (b) with the mixture of metal nanoparticles: 1 – roots, control; 2 – roots, experiment; 3 – leaves, control; 4 – leaves, experiment. Figure 5 Content of zinc in wheat seedling tissues. Zinc content in tissues after treatment of seeds (a) and leaves (b) with the mixture of metal nanoparticles: 1 – roots, control; 2 – roots, experiment; 3 – leaves, control; 4 – leaves. After seed treatment with a mixture of metal nanoparticles with subsequent determination of the content of certain metals in the leaves and roots, we found that the iron content decreased in the roots (44%) and in the leaves (27%), copper content decreased in the roots (17.5%) while in the leaves increased by 12.