data). At present, we can only speculate about the mechanistic basis of the host influence on symbiont physiology. A plausible scenario, however, is that the amount, complexity, and reliability of nutrients provided to the symbionts can affect the symbionts’ evolutionary fate by relaxing or increasing selective pressures on maintaining metabolic versatility. Under
this scenario, a nutrient-rich and stable environment provided by the host sustains genome erosion in the symbiotic bacteria, leading SBI-0206965 ic50 to metabolic dependency and high host specificity (Figure 6). Despite the higher costs, providing a rich environment could be beneficial to the host by stimulating bacterial growth and increasing the number of bacterial cells applied onto the cocoon, which in turn leads to high antibiotic production and an effective symbiont-mediated host protection [35]. Simultaneously,
a rich environment could allow for selection of the best symbionts by ‘screening’ through increased competition, with the most competitive and best-defended strain winning out [36,37]. By contrast, a nutrient-poor environment (lower amount, diversity, and/or reliability of nutrients) would be less costly to the host and prevent genome erosion in the bacterial symbionts. The high metabolic versatility would enable the bacteria to persist as free-living forms and provide the opportunity Ferrostatin-1 mouse for host switching by horizontal transfer (Figure 6). Interestingly, different symbiont strains across individuals of the same host species have so far only been detected for North American Philanthus species ([28], this study: biovar ‘albopilosus’ strains alb539-2), suggesting that horizontal transfer of symbionts is indeed more common among these physiologically versatile strains than across species in the metabolically more restricted South American and Eurasian/African clades. Such horizontal transfer could occur in populations of sympatric host species through interspecific predation or by the acquisition of symbionts from the soil in reused or closely associated brood chambers (Figure 6). Figure 6 Scheme of
putative host-driven evolution within the monophyletic clade ‘ S. philanthi ’. Acquisition of Rucaparib symbionts occurs shortly before or during emergence of the adult female beewolf from the cocoon, and only few bacterial cells are taken up into the antennal gland reservoirs [26]. The strong bottleneck effect likely contributes to the low genetic diversity we observed within the antennae of individual beewolves, as well as across host individuals of the same species (see also [28]). While the genetic homogeneity of the symbionts reduces competition and conflict in the symbiosis, it also compromises the symbionts’ ability to adapt to changing environmental conditions [38]. Furthermore, the uptake of low numbers of symbiont cells from the cocoon surface may entail the risk of taking up non-symbiotic bacteria into the antennae.