Electronic supplementary material Additional file 1: IL-27 did no

Electronic supplementary material Additional file 1: IL-27 did not alter the activation of other signaling pathways. A549 cells were treated with IL-27 (50 ng/mL) for 15 minutes

to 1 hour. The phosphorylated forms of Akt, STAT5, p38 and MAPK/ERK1/2 were detected by Western blot. (PDF 80 KB) References 1. Villarino AV, Huang E, Hunter CA: Understanding the pro- and anti-inflammatory properties of IL-27. J Immunol 2004,173(2):715–720.PubMed 2. Salcedo R, Stauffer JK, Lincoln E, Back TC, Hixon JA, Hahn C, Shafer-Weaver K, Malyguine A, Kastelein R, Wigginton JM: IL-27 mediates complete regression of orthotopic primary and metastatic murine neuroblastoma tumors: role for CD8+ T cells. J Immunol 2004,173(12):7170–7182.PubMed 3. Cocco C, Giuliani N, Di Carlo E, Ognio find more E, Storti P,

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