Few labeled fibers

were seen in the olfactory tubercle, ventral border of the nucleus of the horizontal limb of the diagonal band, fundus striati, caudal accumbens, lateral septal nucleus (Figs. 3A and B), infralimbic cortex and anterior olfactory nucleus. The accessory olfactory bulb is devoid of labeling. Fibers from the posterior BST proceed ventrocaudally in the direction to the hypothalamus to innervate very lightly, at the preoptic anterior hypothalamic level, the rostral part of the medial preoptic nucleus and medial preoptic area (Figs. 3B and C) and, more substantially, the retrochiasmatic area (Fig. 3D). Labeled fibers in the anterior hypothalamic nucleus Selleckchem CDK inhibitor have largely spaced varicosities and seemingly provide only a sparse input to this nucleus (Figs. 3C–E). A few fibers were found

in the periventricular zone including the paraventricular nucleus, which shows a modest number of terminals in the anterior parvo- and magnocellular parts (Figs. 3C and D). At the tuberal level, the MeAV provides a particularly dense input to the selleckchem dorsomedial and central parts of the ventromedial nucleus, but the anterior and ventrolateral parts are also labeled (Figs. 3E–G, 7A). Curiously, the caudal extent of the ventrolateral part, which in Nissl-stained sections shows more densely-packed and darkly-stained neurons than the rest of the ventrolateral part (Coolen et al., 1996), is almost completely avoided. Terminal labeling was also observed in the subfornical region of the lateral hypothalamus, but ventral to it, the tuberal nucleus many is sparsely labeled (Figs. 3F,G, 7A). In addition, a few varicose axons were found in the dorsomedial hypothalamic nucleus and intermediate periventricular nucleus (Figs. 3F–H). At the mammillary level, a rather modest terminal field was observed in the ventral premammillary

nucleus and a lighter one in the ventrolateral part of the dorsal premammillary nucleus (Figs. 3I, J, 7C). Some varicose fibers were also noted in the posterior periventricular nucleus, lateral hypothalamic area (Fig. 3I) and, still fewer, in the posterior hypothalamic nucleus and supramammillary region (Figs. 3I–K, 7C). Some labeled fibers enter the periventricular gray to innervate very lightly the dorsolateral periaqueductal gray (Fig. 3L) and dorsal raphe nucleus. Only occasional fibers were found in the nucleus reuniens, paraventricular nucleus and mediodorsal nucleus of the thalamus. A few labeled fibers cross the midline in the anterior, supraoptic and posterior commissures and in the supramammillary region. The ventromedial hypothalamic nucleus is the sole structure on the contralateral side of the brain which shows an appreciable number of terminals (Figs. 3F–G). The pattern of anterograde labeling observed after injections in the MeAD and MePV is similar to that described in male rats by Canteras et al. (1995).