Forty-six (69.7%)

of 66 male patients were categorized in the low group, whereas only 15 (44.1%) of 34 female patients were categorized in this group. Table 1 Correlation between serum adiponectin level and clinicopathological characteristics in gastric cancer patients.   Adiponectin high group (n = 39) Adiponectin low group (n = 61) p value Age (y) 63.5 ± 12.1 60.6 ± 13.2 0.275 Gender          Male 20 46 0.013    Female 19 15   BMI 22.1 ± 3.6 23.4 ± 3.9 0.079 Macroscopic type          Elevated 5 6 0.642    Depressed/flat 34 55   Depth SBI-0206965 in vitro of Metabolism inhibitor invasion          T1 15 31 0.227    T2, T3 and T4 24 30   Histological type          differentiated 17 22 0.558 Selleckchem PF 01367338    undifferentiated 23 38   Lymphatic invasion          positive 32 42 0.142    negative 7 19   Venous invasion          positive 22 33 0.821    negative 17 28   Lymphatic metastasis          positive 23 34 0.750    negative 16 27   Peritoneal dissemination          positive 9 8 0.196    negative 30 53   Hematogenous metastasis          positive 1 3 0.558    negative 38 58   Stage          I and II 26 41 0.910    III and IV 13 20   AdipoR1/R2 expression in gastric cancer The protein expression of AdipoR1 and AdipoR2 was confirmed by immunostaining of surgically resected gastric cancer tissue specimens (Figure 4). AdipoR1 and AdipoR2 were positively

detected in the cytoplasm as well as the cell membrane of cancer cells. In contrast, normal gastric epithelial cells did not show significant immunoreactivity for either AdipoR1 or AdipoR2. In some parietal cells of normal gastric mucosa, slight reactivity was observed in AdipoR2 expression. This was in accordance with the findings of Ishikawa et al [28]. Figure 4 Representative photomicrographs. Representative photomicrographs of immunohistochemical staining of AdipoR1 (A, normal mucosa; B, cancer tissue)

and AdipoR2 (C, normal mucosa; D, cancer tissue). AdipoR1 and AdipoR2 were expressed in normal gastric mucosa in the cytoplasm as well as in the cell membrane. In gastric cancer tissues, higher intensity of immunostaining compared to normal mucosa was considered positive. Original magnification, ×100. AdipoR1 expression was significantly associated with over histopathological type (p = 0.011) (Table 2). In addition, negative AdipoR1 immunostaining was significantly higher in patients with lymphatic metastasis (p = 0.013; Table 2) and peritoneal dissemination (p = 0.042; Table 2). On the other hand, AdipoR2 expression was also associated with the histopathological type (p = 0.001; Table 3). However, no significant differences were observed in other clinicopathological characteristics (Table 3). Table 2 Expression of AdipoR1 and clinicopathological characteristics in gastric cancer patients.