Functional performance was measured using the standard, indoor, six-minute walk test protocol recommended by the American Thoracic Society (2002). Subjects were instructed to walk along a 30-metre corridor at their own pace for a six-minute
period. This test serves as an indicator of exercise tolerance and symptoms (Olsson et al 2005) and as a prognostic indicator PF-01367338 mouse for subsequent cardiac death (Rostagno et al 2003). We also converted the result to a percentage of the predicted distance on the test for each participant, according to the reference equation of Enright and Sherrill (1998). Disability was measured using the Groningen Activity Restriction Scale, which was administered by face-to-face interview to measure disability in the domains of personal care and domestic activities. It includes 18 items with scores from 1 to NVP-BGJ398 concentration 4, assessing disability in the area of activities of daily living, including mobility and instrumental activities of daily living. The total score can range from 18 (absence of disability) to 72 (highly disabled) (Kempen et al 1996). Health-related quality of life was measured with the Minnesota Living with Heart Failure Questionnaire. It is a validated 21-item disease-specific questionnaire that measures physical, socioeconomic, and psychological impairment related to heart failure. The score is based on how each person ranks each item on a common
scale and it is used to quantify how much heart failure has influenced aspects of a subject’s daily life during the previous month and how it is affected by therapeutic intervention. Scores range from 0 to 105 points, with lower scores indicating less effect from heart failure symptoms and thus a better quality of life (Middel et al 2001, Rector and Cohn, 1992). Group characteristics were analysed with descriptive statistics and are presented as means with standard deviations. Pearson correlation was used to evaluate the bivariate
relationship among the variables at baseline of all the subjects, and also to analyse the relationships between changes in outcome measures for subjects in the experimental group. Group comparisons were tested by two-way repeated measures analysis of variance. For a given outcome without significant group × time interaction, Sclareol analysis of main effect was performed. A p value less than 0.05 was considered as statistically significant. We sought to detect a between-group difference in the change in the Minnesota Living with Heart Failure Questionnaire score of 5 points as this is considered a clinically important improvement in quality of life ( Riegel et al 2002). Assuming that the standard deviation in this score would be similar to that observed in a similar study of exercise in people with chronic heart failure ( Koukouvou et al 2004), a total sample size of 32 would provide 80% power to detect a difference of 5 points as statistically significant.