In order to improve the E.E. of the polymer-Fe3O4 complexes and realize the controlled release of the DNA, we modified the Fe3O4 with multifunctional groups CTS and PEG. In addition, the process of linking polymeric groups did not utilize organic solvent extraction, and the iron content used does not

Inhibitors,research,lifescience,medical surpass the acceptable daily intake. Furthermore, some of the novel nanoparticles could improve the antigen presentation effect, show a better adjuvant effect, and make a long-term, single-immunization vaccine possible [23]. There are likely to be further applicative studies of polymer-Fe3O4 complexes as gene delivery systems. Preliminary Inhibitors,research,lifescience,medical data from our studies suggest that Fe3O4 nanoparticles when decorating with positive-charged polymer CTS exhibit preferential gene delivery. 4. Conclusion CTS-Fe3O4 and PEG-Fe3O4 were successfully prepared. DNA encapsulation efficiency increased

with the proportion of polymer-Fe3O4 nanoparticles, and the optimal E.E. (3:1) was chosen. Simultaneously, Inhibitors,research,lifescience,medical the attachment of DNA to polymer-Fe3O4 complexes did provide protection against cleavage by nuclease. The target distribution of polymer-Fe3O4 complexes with an outer magnetic field was demonstrated in vivo. The controlled-release Inhibitors,research,lifescience,medical effect of CTS-Fe3O4 complexes was more apparent than PEG-Fe3O4, and the DNA binding and release from the polymer-Fe3O4 do not alter its functionality. Both CTS-Fe3O4 and PEG-Fe3O4 had low cytotoxicity to HEK-293 and HepG2 cells. The concentration

of 2mM or less in an in vitro application was shown to be absolutely safe. In addition, the magnetic forces lead to an accelerated sedimentation of polymer-Fe3O4 complexes on the cell surface and do directly enhance the transfection efficiency in HepG2 and SP2/0 Inhibitors,research,lifescience,medical cells compared with conventional transfection methods. The novel gene delivery system proved to be an effective tool for future, and it is promising in targeting expression and delivery of therapeutic genes in in vivo studies. Our study explored why the application of polymer-Fe3O4 nanoparticles as gene carriers. We will continue to do research in this field to provide a more detailed evaluation about the transfer of DNA. Conflict of Interests All of the authors have no conflict of interests. Acknowledgment The authors thank the financial support from National MEK inhibitor Natural Science Foundation of China (Grant no. 30901270).
The ultimate goal of targeted nanotechnology-based drug delivery systems (nanoDDSs) in cancer therapy is to improve the therapeutic index of cytotoxic agents by selectively increasing their concentration at the tumor site.