In Week 0 and Week 16, intake was below 2/3 of the RDA in 42.9% of the participants [29]. Mean carbohydrate intake was below the RDA [28] at all time points, whereas fat and protein intakes were above 100% of the RDA [28]. Table 3 Recommended daily allowance covered for energy, macronutrients and folic acid at three time points Nutrient ≤ 2/3 RDA > 2/3 RDA ≤ RDA > RDA Macronutrients (%) Protein Week 0 – - 100 Week 8 – - 100 Week 16 – - 100 Carbohydrate Week 0 35.7 64.3 – Week 8 – 92.9 7.1 Week 16 – 100 – Fat Week

0 – - 100 Week 8 – - 100 Week 16 – - 100 Vitamins (%) Folic acid Stem Cell Compound Library datasheet Week 0 42.9 42.9 14.3 Week 8 – - 100 Week 16 42.9 50.0 7.1 RDA, recommended daily allowance. Training profile The results in Figure 1 show the training loads recorded during the study period. Training load is reported here as training time, RPE and distribution among three levels of intensity during the intervention (STp) and post-intervention periods (NSTp). There were no statistically significant differences in training time

between STp and NSTp. Figure 1 Comparison of training variables throughout the experimental trial. *Statistically significant difference (P < 0.05) STp vs NSTp. Overall https://www.selleckchem.com/screening/protease-inhibitor-library.html RPE during STp was significantly lower (P < 0.05) than during NSTp. With regard to the durations of different RHR levels (training intensity), a significant difference (P < 0.05) was found for the 60%–80% range, which accounted for 30.35% of the total training time during STp, and for 35.87% of the training time during the NSTp. There were no significant differences for training intensity levels in the <60% range or the >80% range. Bivariate analysis to calculate Pearson’s correlation coefficient detected statistically significant correlations (P < 0.01) between overall RPE and training intensity levels of 60%–80% RHR (r = 0.64) and >80% RHR (r = 0.76). Biochemical assays The results

of biochemical analyses are shown in Table 4. There were no significant changes in plasma folic acid at any time point, and all values were within the normal Amisulpride range for the healthy population. However, plasma concentrations of Hcy increased significantly (P < 0.05) to above the normal range of values during the Week 8 and Week 16 periods compared to baseline values in Week 0. Regarding the relationship between plasma concentrations of Hcy and folic acid and training intensity, we found that both plasma concentrations showed a significant negative correlation (r = −0.75) (P < 0.01) with the level of intensity of <60% RHR. Bivariate analysis disclosed a significant negative correlation (P < 0.01) between Hcy and folic acid concentrations (r = −0.84) in Week 8. Table 4 Biochemical values of clinical and nutritional parameters at three time points N = 14 Study period Biochemical parameters Reference value Week 0 Week 8 Week 16     Mean SD Mean SD Mean SD Transferrin (mg/dl) 200 – 360 261.21 27.82 261.71 33.00 265.50 28.67 Prealbumin (mg/dl) 20 – 40 26.76 3.53 27.