Only one of the spots was more abundant in the biopsies from the tender group. In Exp. A2, samples collected 1 h postmortem from the same animals and muscles as in Exp. A1 were analyzed by Western blotting. In these postmortem samples, the same spot from peroxiredoxin-6 as in Exp. A1 was more abundant in the tender group. In addition, one of the other peroxiredoxin-6 spots was also more abundant in the tender group. To verify the results from Exp. A, biopsies from 14 additional animals were Selleckchem Raf inhibitor analyzed in Exp. B
by Western blotting against stress-70 protein, protein DJ-1, peroxiredoxin-6, and malate dehydrogenase. No significant differences between the tough and tender groups could be observed in selleck screening library these biopsies. However, for peroxiredoxin-6, the tendencies pointed in the same direction as in Exp. A. In conclusion, peroxiredoxin-6 might be a potential protein marker for meat tenderness detectable in biopsies and in samples collected shortly after slaughter. However, more animals are needed to verify the findings in the present study.”
“Undertreatment in patients with primary headaches was evaluated in 600 patients attending 7 headache centres in Lombardy by assessing the rates of acute and prophylactic treatments used before the first visit and the rates of prescription of acute and prophylactic treatments
after the visit at the headache centre. Our results clearly showed that most headache patients are likely to receive suboptimal treatments, confirming the utility of headache centres as well as the need for promoting education of GPs and the development of appropriate networks to reduce undertreatment rates, in order to selleck inhibitor highlight the negative impact caused by primary headache on individuals and on the society.”
“Phenolic compound profile and antioxidant activity of methanol, acetone, ethyl acetate, water and petroleum ether (b.p. 40-60 degrees C) extracts of Dorystoechas hastata, endemic to Turkey and being consumed as herbal tea by local inhabitants, have been investigated. HPLC-DAD analysis indicated the presence of chlorogenic, caffeic, p-coumaric,
ferulic and rosmarinic acids as phenolic acids, quercetin, kaempferol and apigenin as flavonoids and carnosic acid and carnosol as diterpenoid antioxidants in the plant. Petroleum ether, methanol and water extracts of D. hastata were found to be very effective antioxidative extracts. Petroleum ether extract, having the highest amount of carnosic acid and carnosol contents (531.3 and 389.9 mg/g OW, respectively) among all solvent extracts, was found to have the highest antioxidative potential based on the individual antioxidant activity assays; DPPH center dot, ABTS center dot(+), TBARS and total phenolic content, expressed as IC50, TEAC, EC50 and TPC values, respectively, and also on the principal component analysis. It exhibited significantly high TEAC (7.1 mM trolox) and low EC50 (54.