Patients had been treated with chemotherapy, a combination of platinum (carboplatin, cisplatin) and taxanes (taxol, docetaxel) following optimal debulking or cyto-reductive surgery. Available demographic characteristics included age at diagnosis and race, and clinicopathologic characteristics including tumor stage, cell type and grade, optimality of
the primary debulking operation, chemotherapy regimen, number of chemotherapies, disease recurrence, and response of tumors to chemotherapy. The optimal debulking or cyto-reductive surgery is defined INCB024360 as the largest residual tumor nodule measuring 1 cm or less, according to the Gynecologic Oncology Group [19]. The response evaluation criteria in solid tumors (RECIST) [20] were used to define the response of tumors to treatment. Overall survival (OS) and progression-free survival (PFS) were calculated as the date of disease diagnosis to the date of death or last contact or the date of recurrence or progression, accordingly. BYL719 mouse Disease recurrence was defined as the reappearance of any lesion that had previously disappeared or the appearance of a new lesion that was histopathologically
confirmed by a biopsy. Information about the date of last contact and status of patients at the last contact was obtained from the M. D. Anderson Tumor Registry and Social Security Death Index, when this information was missing from the medical records. This study was approved by the M.D. Anderson Institutional Review Board. SNP Selection and Genotyping Using SULF1 gene position from International HapMap project http://hapmap.ncbi.nlm.nih.gov/cgi-perl/gbrowse/hapmap28_B36/#search with the extension of 2 kb at both sides to cover near gene regions (chr8:70539427..70737701), we found that five of 355 SNPs were common in HapMap Caucasian population with one of following predicted functionalities at the SNP Function Prediction website http://snpinfo.niehs.nih.gov/snpfunc.htm: (1)
affecting transcription factor binding sites (TFBS) activity in the putative promoter region, (2) affecting splicing activity, or (3) affecting the microRNA binding sites activity. Therefore, we genotyped all of these five SNPs: rs2623047 G>A, rs13264163 A>G, rs6990375 G>A, rs3802278 G>A, and rs3087714 C>T. The genotyping was Branched chain aminotransferase performed by the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP) using genomic DNA. Table 1 shows the primers and PCR information for each SNP. The PCR conditions consisted of an initial melting step of 95°C for 5 min, followed by 35 cycles of denaturation (95 °C for 30 seconds), annealing (52 – 55 °C for 45 sec according to SNPs), and extension (72°C for 1 min), and a final extension step of 72°C for 10 min. The digested products were checked on a 3% MetaPhor agarose gel containing ethidium bromide.