Promoter sequences within flanking insertion sequences likely influence the expression of many of these BVD-523 supplier resistance genes. Interestingly, the majority of the genomes harbour mutations in gyrA and/or parC genes. Table

2 Antimicrobial resistance gene products encoded by A.baumannii Crenigacestat in vitro genomes Gene Products       Strains         AB0057 AYE 3990 ACICU 4190 ATCC17978 3909 Class C β-lactamase 9, 2796 1110 2437 2564 2076 2367 1404 Class A β-lactamase 283 (TEM-1) – - – - – -   – 3623 (VEB-1) – - – - – Class D β-lactamase 1757 (oxa-69) 2122 (oxa-69) 2827 (oxa-66) 1560 (oxa-66) 63 (oxa-64) 1517 (oxa-95) 1089 (oxa-90)   – - 3514 (oxa-20) 0226

(oxa-20) – - –   0551 (oxa-23)* – - – - – -   – - p2ABST2 (oxa-58)* pACICU1 (oxa-58)* (2X) p1ABST25 (oxa-72)* – p1ABST78 (oxa-58)*   – - – - p2ABST25 (oxa-72)* – - AAC (3)-I aminoglycoside acetyltransferase GSK2879552 concentration 291 3573 – - – - – AAC (6′)-I aminoglycoside acetyltransferase – 3630 3516 223 – - – APH (3′)-I aminoglycoside phosphotransferase 288 3578 – - – - –   – - 3897 1948 560 – - ANT (3”)-I aminoglycoside adenylyltransferase 293 3570,3618 – - 3268 – -   171 3739 1641 156 2954 131 2919 Chloramphenicol acetyl transferase 280 3587 – - – - –   3104 798 3709 2932 1731 2691 1443 DNA topoisomerase II 3037 [R1] 0867 [R1] 0747 [R1] 2869 [R1] 2907 [R1] 2626 [S] 0539 [R1] DNA topoisomerase IV 0232 [R2] 3679 [R2] 1415 [S] 0214 [S] 2382 [R2] – 3413 [R2] RNA polymerase β Subunit 0369 [S] 3489 [S] 2179 [R3] 0303 [S] 3155 [S] 0287 [S] 0411 [S] Dihydropteroate synthase 265, 294 3568,3616,3612 3142 228 – 675 –   Beta adrenergic receptor kinase 3095 807 3700 2923 2684 2680 1433 Dihydrofolate reductase type 1 – 3644 – - – - – Dihydrofolate reductase type 3 540 3315 3351 467 3501 457 403 R, resistant; S, susceptible; R1 81 Ser →Leu; R2 84 Ser → Leu; R 3 535 His → Leu; * carbapenem-hydrolysing class D beta-lactamase; + ORFs

identified by tBLASTn. Shared synteny lets to represent the A. baumannii chromosomes as ˜4 Mb long DNA segments homologous to each other throughout their lengths (Figure 1). DNA tracts, ranging in size from 4 to 126 kb, are present in one or more strains, but missing or replaced by alternative DNA segments in others (see vertical bars in Figure 1). Some of these regions correspond to DNA sequences earlier suspected to be mobile because found in A. baumannii but not in A. baylyi DNA or vice versa [17, 27]. Specific 15-36 kb regions are missing in all strains but AB0057 (see triangles in Figure 1), and may therefore plausibly correspond to strain-specific deletions.