PubMed

PubMedCrossRef 39. Shaw DM, Gaerthé B, Leer RJ, Van der Stap JG, Smittenaar C, Heijne Den Bak-Glashouwer M, Thole JR, Tielen FJ, Pouwels PH, Havenith CE: Engineering the microflora to vaccinate the mucosa: serum immunoglobulin G responses and activated draining cervical lymph nodes following mucosal application of tetanus toxin fragment C-expressing lactobacilli . Immunology 2000, 100:510–518.PubMedCrossRef 40. Xin KQ, Hoshino Y, Toda Y, Igimi S, Kojima Y, Jounai

N, Ohba K, Kushiro A, Kiwaki M, Hamajima K, Klinman D, Okuda K: Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV. Env Blood 2003, 102:223–228.CrossRef 41. Fagarasan S, Honjo T: Intestinal IgA synthesis: regulation of front-line body defences. Nat Rev Immunol 2003, 3:63–72.PubMedCrossRef 42. Sambrook J, Fritisch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. 3 Edition New York: Cold Spring Harbor Laboratory GDC-941 2001. 43. Sambrook J, Fritisch EF, Maniatis T: Molecular cloning: a laboratory manual. 2 Edition Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1989. 44. Shifang J, Yinyu W, Xinhua G, Liandong H: The factors affected Mizoribine datasheet transformation efficiency of Lactobacillus by electroporation. selleck screening library Chin J Biotechnol 1998, 14:429–33. 45. Cortes-Perez NG, Luis G: Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein. FEMS Microbiol Lett 2003, 229:37–42.PubMedCrossRef

46. McCluskie MJ, Davis HL: CpG DNA is a potent enhancer of systemic and mucosal immune responses against hepatitis B surface antigen with intranasal administration to mice. J Immunol 1998, 161:4463–4466.PubMed 47. Ho PS, Wang JK, Lee YK: Intragastric administration of Lactobacillus

casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 2005, 23:1335–42.PubMedCrossRef Authors’ contributions XQ carried out construction of expression plasmid, participated in the sequence alignment and drafted the manuscript. GL carried out the protein expression and immunoassays. XW and XL carried out the Immunizations. ML performed the statistical Montelukast Sodium analysis. YL conceived of the study, and participated in its design and coordination. All authors read and approved the final manuscript.”
“Background The Atlantic cod (Gadus morhua) is a cold-adapted fish species which has been captured for human consumption for many years. It is a perishable commodity and for that reason, preservation methods like freezing or salting have traditionally been used to extend its shelf life [1, 2]. The fish is a microbial ecosystem of its own where the ecological principles of succession are as valid as in any other ecosystem. This microbiological environment consists of a high nutrient content with an oxygen tension favourable to the proliferation of fast-growing heterotrophs also responsible for the spoilage of food [3–5].

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