Serum alphafetoprotein (AFP) level over 100 ng/mL during hepatitis flare usually represents more extensive hepatocytolysis or bridging hepatic necrosis (Liver 1986; 6:133-7). Whether higher AFP levels during hepatitis flare are associated with greater reduction in HBsAg level during Nuc therapy is unknown. Patients and methods: The study included 217 chronic hepatitis B patients who had adequate AFP measurement during hepatitis B flare (ALT ≥ 5X ULN). HBsAg level was measured at baseline, 6 month and 12 month of Nuc therapy using Elecsys HBsAg II (Roche Diagnostics, Indianapolis, IN, USA). The

PCI-32765 nmr results were analyzed according to AFP levels (<20, 20-50, 50-100 and ≥ 100 ng/mL) and ALT levels (5-10X, 10-20X, > 20XULN), respectively, and compared with that of 44 CHB patients with ALT < 5X ULN. Results: The results (Table 1) showed the higher the AFP level during hepatitis flare, the greater the reduction in HBsAg level during Nuc therapy. The reduction in HBsAg level was also greater in patients with higher ALT levels. Stepwise multiple linear regression analysis showed that AFP level, not ALT level, was significantly associated with greater reduction in 6th month and 1st year

HBsAg levels. Conclusion: The increase of AFP during hepatitis B flare, reflecting more extensive hepatocytolysis and subsequent regeneration, contributes to greater reduction in HBsAg level during Nuc therapy. Log10 reduction in HBsAg level in patients with VX-809 mouse different AFP and ALT levels Linear trend of HBsAg Log reduction at 6th month by ALT and AFP are P=0.000, respectively. Linear trend of HBsAg Log reduction at 12th month by ALT and AFP are P=0.000, respectively. AFP: alphafetoprotein; ALT: alanine transaminase; ULN: upper limit of normal; HBsAg: hepatitis B surface antigen. Disclosures: Yun -Fan Liaw -

Advisory Committees or Review Panels: Roche; Grant/Research Support: Roche The following people have nothing to disclose: Rachel Wen-Juei medchemexpress Jeng, Yi-Cheng Chen, Ming-Ling Chang Background and Aim: Liver Stiffness (LS) measured by Fibros-can (transient elastography; TE) has been reported to correlate with fibrosis stages in various liver diseases. The purpose of antiviral treatment for chronic hepatitis B is a suppression of liver fibrosis progression and a reduction of the risk for hepato-cellular carcinoma (HCC). The aim of the present study was to evaluate the effect of antiviral treatment on LS and its correlation to hepatocarcinogenesis in chronic hepatitis B. Methods: LS (kPa) was measured by TE in 372 patients with chronic hepatitis B.